Wheat germ agglutinin (WGA): a bovine T lymphocyte marker

Vet Immunol Immunopathol. 1987 Sep;16(1-2):151-4. doi: 10.1016/0165-2427(87)90181-4.

Abstract

Bovine peripheral blood lymphocytes (PBL) were examined for their ability to bind wheat germ agglutinin (WGA). This lectin labelled 43.8% +/- 11.95 of bovine PBL, whereas peanut agglutinin (PNA), a T cell marker, bound 59.4% +/- 8.67 cells, and surface immunoglobulin (SLG)-bearing cells constituted 24.15% +/- 8.47 of PBL. After panning fractionation of B (Slg+) and T (PNA+) lymphocytes. WGA labelled 89 to 97% of the enriched T cell population (80/87% PNA+; 2-4% Slg+) but only 6 to 8% of the enriched B cell population (85-91% Slg+; 5-7% PNA+).

MeSH terms

  • Animals
  • B-Lymphocytes / metabolism
  • Binding Sites
  • Cattle
  • In Vitro Techniques
  • T-Lymphocytes / metabolism*
  • Wheat Germ Agglutinins / metabolism*

Substances

  • Wheat Germ Agglutinins