Preparation and antibacterial properties of an AgBr@SiO2/GelMA composite hydrogel

Biomed Mater. 2022 Jan 25;17(2). doi: 10.1088/1748-605X/ac49f7.

Abstract

Pure gelatin hydrogels lack antibacterial function and have poor mechanical properties, which restrict their application in wound dressings. In this study, nanosized silver bromide-doped mesoporous silica (AgBr@SiO2) microspheres with hollow structures were prepared by a modified Stober method. The novel microspheres can not only release silver ions to treat bacteria but also release drugs to treat skin wound. Furthermore, AgBr@SiO2microspheres were modified with propyl methacrylate, incorporated into methacrylated gelatin (GelMA), and crosslinked by UV light to prepare AgBr@SiO2/GelMA dressings consisting of composite hydrogels. The results showed that the AgBr@SiO2microspheres could enhance the mechanical properties of the hydrogels. With the increase in the AgBr@SiO2concentration from 0.5 to 1 mg ml-1, the dressings demonstrated effective antimicrobial activity against bothStaphylococcus aureusandEscherichia coli. Furthermore, full-thickness skin woundsin vivowound healing studies with Sprague-Dawley rats were evaluated. When treated with AgBr@SiO2/GelMA containing 1 mg ml-1AgBr@SiO2, only 15% of the wound area left on day 10. Histology results also showed the epidermal and dermal layers were better organized. These results suggest that AgBr@SiO2/GelMA-based dressing materials could be promising candidates for wound dressings.

Keywords: AgBr nanoparticles; antibacterial; hydrogel; methacrylic anhydride; wound dressing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Bacteria / drug effects
  • Bandages
  • Bromides* / chemistry
  • Bromides* / pharmacology
  • Female
  • Gelatin / chemistry
  • Gelatin / pharmacology
  • Hydrogels* / chemistry
  • Hydrogels* / pharmacology
  • Metal Nanoparticles / chemistry*
  • Microspheres
  • Rats
  • Rats, Sprague-Dawley
  • Silicon Dioxide / chemistry
  • Silicon Dioxide / pharmacology
  • Silver Compounds* / chemistry
  • Silver Compounds* / pharmacology
  • Wound Healing / drug effects

Substances

  • Anti-Bacterial Agents
  • Bromides
  • Hydrogels
  • Silver Compounds
  • Silicon Dioxide
  • Gelatin
  • silver bromide