RNA modifications are crucial for the proper function of the RNAs. The sites of pseudouridines are often specified by dual hairpin guide RNAs, with one or both hairpins identifying a target uridine. In this issue of Genes & Development, Jády and colleagues (pp. 70-83) identify a novel mechanism by which a single guide RNA hairpin can specify two uridines adjacent to each other or separated by 1 nt; i.e., one for two or guide RNA acrobatics.
Keywords: RNA-guided RNA modification; RNA–RNA interaction; box H/ACA RNAs; guide RNA acrobatics; pseudouridine; pseudouridylation.
© 2022 Meier; Published by Cold Spring Harbor Laboratory Press.