Tolerogenic nanoparticles mitigate the formation of anti-drug antibodies against pegylated uricase in patients with hyperuricemia

Nat Commun. 2022 Jan 12;13(1):272. doi: 10.1038/s41467-021-27945-7.

Abstract

Biologic drugs have transformed the standard of care for many diseases. However, many biologics induce the formation of anti-drug antibodies (ADAs), which can compromise their safety and efficacy. Preclinical studies demonstrate that biodegradable nanoparticles-encapsulating rapamycin (ImmTOR), but not free rapamycin, mitigate the immunogenicity of co-administered biologic drugs. Here we report the outcomes from two clinical trials for ImmTOR. In the first ascending dose, open-label study (NCT02464605), pegadricase, an immunogenic, pegylated uricase enzyme derived from Candida utilis, is assessed for safety and tolerability (primary endpoint) as well as activity and immunogenicity (secondary endpoint); in the second single ascending dose Phase 1b trial (NCT02648269) composed of both a double-blind and open-label parts, we evaluate the safety of ImmTOR (primary endpoint) and its ability to prevent the formation of anti-drug antibodies against pegadricase and enhance its pharmacodynamic activity (secondary endpoint) in patients with hyperuricemia. The combination of ImmTOR and pegadricase is well tolerated. ImmTOR inhibits the development of uricase-specific ADAs in a dose-dependent manner, thus enabling sustained enzyme activity and reduction in serum uric acid levels. ImmTOR may thus represent a feasible approach for preventing the formation of ADAs to a broad range of immunogenic biologic therapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibodies / chemistry
  • Antibodies / therapeutic use*
  • Biological Therapy
  • Double-Blind Method
  • Female
  • Humans
  • Hyperuricemia / drug therapy*
  • Male
  • Middle Aged
  • Nanoparticles / chemistry*
  • Nanoparticles / therapeutic use*
  • Organic Chemicals / chemistry
  • Polyethylene Glycols / pharmacology*
  • Urate Oxidase / pharmacology
  • Urate Oxidase / therapeutic use*
  • Uric Acid
  • Young Adult

Substances

  • Antibodies
  • Organic Chemicals
  • Tolerogen
  • Uric Acid
  • Polyethylene Glycols
  • Urate Oxidase

Associated data

  • ClinicalTrials.gov/NCT02464605
  • ClinicalTrials.gov/NCT02648269