Recombinase polymerase amplification assay for sensitive and rapid detection of southern rice black-streaked dwarf virus in plants

Wendi Ma; Xueyan Duan; Yiying Tian; Zhiyuan Jiao; Xi Sun; Zhenxing Zhao; Tao Zhou; Zaifeng Fan

doi:10.1016/j.jviromet.2022.114467

Recombinase polymerase amplification assay for sensitive and rapid detection of southern rice black-streaked dwarf virus in plants

J Virol Methods. 2022 Mar:301:114467. doi: 10.1016/j.jviromet.2022.114467. Epub 2022 Jan 13.

Authors

Wendi Ma¹, Xueyan Duan², Yiying Tian³, Zhiyuan Jiao⁴, Xi Sun⁵, Zhenxing Zhao⁶, Tao Zhou⁷, Zaifeng Fan⁸

Affiliations

¹ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].
² State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].
³ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].
⁴ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].
⁵ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].
⁶ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China; Chinese Academy of Inspection and Quarantine, Beijing, China. Electronic address: [email protected].
⁷ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].
⁸ State Kay Laboratory of Agrobiotechnology and Key Laboratory of Pest Monitoring and Green Management-MARA, Department of Plant Pathology and Sanya Institute of China Agricultural University, Beijing, 100193, China. Electronic address: [email protected].

PMID: 35033578
DOI: 10.1016/j.jviromet.2022.114467

Abstract

Southern rice black-streaked dwarf virus (SRBSDV) naturally infects rice and maize plants through white-backed planthopper (Sogatella furcifera) causing significant crop losses in China and Vietnam. Thus, rapid and accurate detection methods for SRBSDV are urgently needed. Recombinase polymerase amplification (RPA) is a novel technique for rapid and sensitive detection of nucleic acids. In this research, a reverse transcription (RT)-RPA-based method was developed for the detection of SRBSDV. A pair of RPA primers targeting the conserved sequences within the SP10 (major coat protein) gene on genomic RNA S10 of SRBSDV were designed. The assay was performed in a single tube at 39 °C for 20 min and demonstrated that the RPA assay is an efficient alternative for rapid detection of SRBSDV.

Keywords: Fijivirus; RPA; Virus detection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Oryza*
Plant Diseases
Recombinases
Reoviridae* / genetics

Substances

Recombinases

Supplementary concepts

Southern rice black-streaked dwarf virus