Hypozincemia in COVID-19 Patients Correlates With Stronger Antibody Response

Front Immunol. 2022 Jan 4:12:785599. doi: 10.3389/fimmu.2021.785599. eCollection 2021.

Abstract

Zinc ion as an enzyme cofactor exhibits antiviral and anti-inflammatory activity during infection, but circulating zinc ion level during Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is unclear. This study aimed to evaluate serum zinc ion level in Coronavirus Disease 2019 (COVID-19) patients and healthy subjects, as well as its correlation with antibodies against SARS-CoV-2. 114 COVID-19 patients and 48 healthy subjects (38 healthy volunteers and 10 close contacts of patients with COVID-19) were included. Zinc ion concentration and levels of antibodies against SARS-CoV-2 Spike 1 + Spike 2 proteins, nucleocapsid protein, and receptor-binding domain in serum were measured. Results showed that the concentration of zinc ion in serum from COVID-19 patients [median: 6.4 nmol/mL (IQR 1.5 - 12.0 nmol/mL)] were significantly lower than that from the healthy subjects [median: 15.0 nmol/mL (IQR 11.9 - 18.8 nmol/mL)] (p < 0.001) and the difference remained significant after age stratification (p < 0.001) or when the patients were at the recovery stage (p < 0.001). Furthermore, COVID-19 patients with more severe hypozincemia showed higher levels of IgG against the receptor-binding domain of SARS-CoV-2 spike protein. Further studies to confirm the effect of zinc supplementation on improving the outcomes of COVID-19, including antibody response against SARS-CoV-2, are warranted.

Keywords: COVID - 19; RBD; SARS – CoV – 2; antibody; zinc.

MeSH terms

  • Adult
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • COVID-19 / blood*
  • COVID-19 / immunology*
  • COVID-19 / virology
  • Case-Control Studies
  • Cations, Divalent / blood
  • Coronavirus Nucleocapsid Proteins / immunology
  • Female
  • Humans
  • Immunity*
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Male
  • Middle Aged
  • Phosphoproteins / immunology
  • Protein Domains / immunology
  • Real-Time Polymerase Chain Reaction / methods
  • Retrospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • SARS-CoV-2 / genetics
  • SARS-CoV-2 / immunology*
  • Spike Glycoprotein, Coronavirus / immunology
  • Zinc / blood*

Substances

  • Antibodies, Viral
  • Cations, Divalent
  • Coronavirus Nucleocapsid Proteins
  • Immunoglobulin G
  • Phosphoproteins
  • Spike Glycoprotein, Coronavirus
  • nucleocapsid phosphoprotein, SARS-CoV-2
  • spike protein, SARS-CoV-2
  • Zinc