A highly selective 2''- O-glycosyltransferase from Ziziphus jujuba and De novo biosynthesis of isovitexin 2''- O-glucoside

Ya-Qun Zhang; Zi-Long Wang; Zhuo Chen; Zheng-Tong Jin; Aobulikasimu Hasan; Hai-Dong Wang; Yu-Wei Sun; Xue Qiao; Yong Wang; Min Ye

doi:10.1039/d1cc06949g

A highly selective 2''- O-glycosyltransferase from Ziziphus jujuba and De novo biosynthesis of isovitexin 2''- O-glucoside

Chem Commun (Camb). 2022 Feb 17;58(15):2472-2475. doi: 10.1039/d1cc06949g.

Authors

Ya-Qun Zhang¹, Zi-Long Wang¹, Zhuo Chen^{2

3}, Zheng-Tong Jin¹, Aobulikasimu Hasan¹, Hai-Dong Wang¹, Yu-Wei Sun², Xue Qiao¹, Yong Wang², Min Ye¹

Affiliations

¹ State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. [email protected].
² CAS-Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China. [email protected].
³ University of Chinese Academy of Sciences, Beijing 100039, China.

PMID: 35084410
DOI: 10.1039/d1cc06949g

Abstract

A novel and efficient 2''-O-glycosyltransferase ZjOGT38 was identified from Ziziphus jujuba. It could regio-selectively glycosylate 2-hydroxyflavanone C-glycosides. ZjOGT38 allowed de novo biosynthesis of isovitexin 2''-O-glucoside in E. coli.

MeSH terms

Glucosides / biosynthesis*
Glucosides / chemistry
Glycosyltransferases / metabolism*
Isoflavones / biosynthesis*
Isoflavones / chemistry
Molecular Structure
Ziziphus / enzymology*

Substances

Glucosides
Isoflavones
isovitexin 2''-O-glucoside
Glycosyltransferases