Establishment of SIAISi017-A, an induced pluripotent stem cell(iPSC)line from a healthy 25-year-old Chinese Hui

Yi Zhang; Jiajun Shou; Wentao Gu; Weihao Di; Jian Zhao; Ying Wang; Shixin Gu

doi:10.1016/j.scr.2021.102645

Establishment of SIAISi017-A, an induced pluripotent stem cell(iPSC)line from a healthy 25-year-old Chinese Hui

Stem Cell Res. 2022 Apr:60:102645. doi: 10.1016/j.scr.2021.102645. Epub 2022 Jan 20.

Authors

Yi Zhang¹, Jiajun Shou¹, Wentao Gu¹, Weihao Di², Jian Zhao², Ying Wang², Shixin Gu¹

Affiliations

¹ Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China.
² Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, Shanghai 201210, China.

PMID: 35093714
DOI: 10.1016/j.scr.2021.102645

Abstract

With the development of cytology, the establishment of cell models in vitro has become a powerful means to study the mechanism and treatment of diseases. Here we successfully generated the IPSC-derived modeling system of a 25-year-old healthy male. His peripheral blood mononuclear cells (PBMC) were reprogrammed using human OKSM (SOX2, OCT3/4, KLF4, and C-MYC) transcription factors using a non-integrated additional vector system. Immunocytochemistry demonstrated that IPSCS expressed all the markers of pluripotency and demonstrated their ability to differentiate spontaneously from three hypoderms in vitro. Karyotype is normal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Asian People
Cell Differentiation
China
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Male
Transcription Factors / genetics

Substances

Transcription Factors