Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment

Ignacio Mastandrea; Divsha Sher; Prerna Magod; Dinorah Friedmann-Morvinski

doi:10.1016/j.xpro.2021.101106

Isolation and characterization of the immune cell fraction from murine brain tumor microenvironment

STAR Protoc. 2022 Jan 20;3(1):101106. doi: 10.1016/j.xpro.2021.101106. eCollection 2022 Mar 18.

Authors

Ignacio Mastandrea¹, Divsha Sher¹, Prerna Magod¹, Dinorah Friedmann-Morvinski^{1

2}

Affiliations

¹ The School of Neurobiology, Biochemistry and Biophysics, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel.
² Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 6997801, Israel.

Abstract

The immune fraction of the tumor microenvironment has been proven to play a fundamental role in glioblastoma progression and therapeutic response. Here, we present a detailed magnetic-bead-enrichment-based protocol to isolate and analyze the composition of this fraction from mouse brain tumors. The protocol is optimized to achieve high yields of viable immune cells. We also detail characterization of the immune subtypes by FACS analysis. Our procedure is applicable for either lentiviral-induced tumors or transplant models in syngeneic immunocompetent mice. For complete details on the use and execution of this protocol, please refer to Magod et al. (2021).

Keywords: Cancer; Cell Biology; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Neoplasms / immunology*
Brain Neoplasms / pathology
Disease Models, Animal
Flow Cytometry
Mice
Tumor Microenvironment*