Using the Merlin assay for reducing sentinel lymph node biopsy complications in melanoma: a retrospective cohort study

Int J Dermatol. 2022 Jul;61(7):848-854. doi: 10.1111/ijd.16056. Epub 2022 Jan 31.

Abstract

Background: The assessment of the sentinel lymph node is a cornerstone of melanoma staging. However, ~80% of sentinel lymph node biopsies (SLNB) are negative and nontherapeutic, and patients are unnecessarily exposed to surgery-related complications. Here, we gauged the potential of the Merlin assay to reduce SLNB-associated complications. The Merlin assay uses clinicopathologic variables and tumor gene expression profiling to identify low-risk patients who may forgo SLNB.

Methods: We utilized the Merlin test development cohort to determine SLNB complication rates for procedures performed between 2004 and 2018 at Mayo Clinic. Complications evaluated were lymphedema, seroma, infection/cellulitis, hematoma, and wound dehiscence. Patients who underwent a completion lymph node dissection were excluded.

Results: A total of 558 patients were included. The overall 90-day complication rate specific to SLNB (1 year for lymphedema) was 17.4%. The most common complications were seroma (9.3%), infection/cellulitis (4.8%), and lymphedema (4.3%). All three were more common in patients with a lower extremity primary tumor location versus other locations. With Merlin test results applied, SLNB-related complications would have decreased by 59%.

Conclusion: SLNB is a safe procedure but carries a significant complication rate. Merlin testing might reduce the need for SLNB and its associated complications.

MeSH terms

  • Cellulitis
  • Humans
  • Lymph Node Excision / adverse effects
  • Lymph Node Excision / methods
  • Lymphedema* / etiology
  • Melanoma* / pathology
  • Neurofibromin 2
  • Retrospective Studies
  • Sentinel Lymph Node Biopsy / adverse effects
  • Seroma / etiology
  • Skin Neoplasms* / pathology
  • Syndrome

Substances

  • Neurofibromin 2