Orthogonal protocols for DNA extraction from filamentous fungi

Benjamin H Conlon; Suzanne Schmidt; Michael Poulsen; Jonathan Z Shik

doi:10.1016/j.xpro.2022.101126

Orthogonal protocols for DNA extraction from filamentous fungi

STAR Protoc. 2022 Jan 24;3(1):101126. doi: 10.1016/j.xpro.2022.101126. eCollection 2022 Mar 18.

Authors

Benjamin H Conlon¹, Suzanne Schmidt¹, Michael Poulsen¹, Jonathan Z Shik^{1

2}

Affiliations

¹ Section for Ecology and Evolution, Department of Biology, University of Copenhagen, København, Denmark.
² Smithsonian Tropical Research Institute, Apartado Postal 0843-03092, Balboa, Ancon, Panama.

Abstract

There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).

Keywords: Genetics; Genomics; Microbiology; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota* / genetics
Basidiomycota*