Spatio-temporal patterns of Aspergillus flavus infection and aflatoxin B1 biosynthesis on maize kernels probed by SWIR hyperspectral imaging and synchrotron FTIR microspectroscopy

Yao Lu; Beibei Jia; Seung-Chul Yoon; Hong Zhuang; Xinzhi Ni; Baozhu Guo; Scott E Gold; Jake C Fountain; Anthony E Glenn; Kurt C Lawrence; Haicheng Zhang; Xiaohuan Guo; Feng Zhang; Wei Wang

doi:10.1016/j.foodchem.2022.132340

Spatio-temporal patterns of Aspergillus flavus infection and aflatoxin B₁ biosynthesis on maize kernels probed by SWIR hyperspectral imaging and synchrotron FTIR microspectroscopy

Food Chem. 2022 Jul 15:382:132340. doi: 10.1016/j.foodchem.2022.132340. Epub 2022 Feb 3.

Authors

Yao Lu¹, Beibei Jia², Seung-Chul Yoon³, Hong Zhuang³, Xinzhi Ni⁴, Baozhu Guo⁴, Scott E Gold⁵, Jake C Fountain⁶, Anthony E Glenn⁵, Kurt C Lawrence³, Haicheng Zhang¹, Xiaohuan Guo¹, Feng Zhang², Wei Wang⁷

Affiliations

¹ Beijing Key Laboratory of Optimization Design for Modern Agricultural Equipment, College of Engineering, China Agricultural University, Beijing 100083, China.
² Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
³ Quality & Safety Assessment Research Unit, U. S. National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA 30605, USA.
⁴ Crop Genetics and Breeding Research Unit, USDA-ARS, 2747 Davis Road, Tifton, GA 31793, USA.
⁵ Toxicology & Mycotoxin Research Unit, U. S. National Poultry Research Center, USDA-ARS, 950 College Station Rd., Athens, GA 30605, USA.
⁶ Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State, University, Starkville, MS 39762, USA.
⁷ Beijing Key Laboratory of Optimization Design for Modern Agricultural Equipment, College of Engineering, China Agricultural University, Beijing 100083, China. Electronic address: [email protected].

PMID: 35139463
DOI: 10.1016/j.foodchem.2022.132340

Abstract

The dynamics mechanisms regulating the growth and AFB₁ production of Aspergillus flavus during its interactions with maize kernels remain unclear. In this study, shortwave infrared hyperspectral imaging (SWIR-HSI) and synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy were combined to investigate chemical and spatial-temporal changes in incremental damaged maize kernels induced by A. flavus infection at macroscopic and microscopic levels. SWIR-HSI was employed to extract spectral information of A. flavus growth and quantitatively detect AFB₁ levels. Satisfactory full-spectrum models and simplified multispectral models were obtained respectively by partial least squares regression (PLSR) for three types of samples. Furthermore, SR-FTIR microspectroscopy coupled with two-dimensional correlation spectroscopy (2DCOS) was utilized to reveal the possible sequence of dynamic changes of nutrient loss and trace AFB₁ in maize kernels. It exhibited new insights on how to quantify the spatio-temporal patterns of fungal infection and AFB₁ accumulation on maize and provided theoretical basis for online sorting.

Keywords: 2DCOS; AFB(1); Aspergillus flavus; Hyperspectral imaging; Interactive mechanism; SR-FTIR.

MeSH terms

Aflatoxin B1*
Aspergillus flavus*
Hyperspectral Imaging
Spectroscopy, Fourier Transform Infrared
Synchrotrons
Zea mays / chemistry

Substances

Aflatoxin B1