Hydrogen sulfide attenuates intracellular oxidative stress via repressing glycolate oxidase activities in Arabidopsis thaliana

Background: Hydrogen sulfide (H₂S) has been proposed to exert anti-oxidative effect under many environmental stresses; however, how it influences oxidative stress remains largely unclear.

Results: Here, we assessed the effects of H₂S on oxidative stress responses such as salicylic acid (SA)-dependent cell death, which triggered by increased H₂O₂ availability in Arabidopsis thaliana catalase-deficient mutants cat2 displaying around 20% wild-type catalase activity. H₂S generation and its producing enzyme _L-cysteine desulfhydrase (LCD/DES) were found to transient increase in response to intracellular oxidative stress. Although introducing the mutation of des1, an important LCD, into the cat2 background produced little effect, H₂S fumigation not only rescued the cell death phenotype of cat2 plant, but also attenuated SA accumulation and oxidation of the glutathione pool. Unexpectedly, the activities of major components of ascorbate-glutathione pathway were less affected by the presence of H₂S treatment, but decreased glycolate oxidase (GOX) in combination with accumulation of glycolate implied H₂S treatment impacts the cellular redox homeostasis by repressing the GOX-catalyzed reaction likely via altering the major GOX transcript levels.

Conclusions: Our findings reveal a link between H₂S and peroxisomal H₂O₂ production that has implications for the understanding of the multifaceted roles of H₂S in the regulation of oxidative stress responses.