Abstract
We have developed an immunofluorescent screening method in which animal or plant cells are immobilized without fixation on polycarbonate (Nuclepore) membranes. The technique does not involve centrifugation, and virtually no cells are lost during processing. Direct microscopic analysis of antibody binding provides an extremely sensitive and reproducible screening system for hybridoma supernatants.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / analysis*
-
Antibodies, Monoclonal / immunology
-
Antigens, Surface / immunology*
-
Cell Adhesion
-
Cell Membrane / immunology
-
Cell Survival
-
Fluorescent Antibody Technique
-
Membranes, Artificial
-
Plants
-
Polycarboxylate Cement
Substances
-
Antibodies, Monoclonal
-
Antigens, Surface
-
Membranes, Artificial
-
Polycarboxylate Cement
-
polycarbonate