MTT assay based inhibition analysis of A2780 cells proliferation using Mollugo nudicaulis Lam. extract with CXCR4 and HER2 expression

Chella Perumal Palanisamy; Shazia Fathima Jh; Ramya Sekar; Ponnulakshmi Rajagopal; Selvaraj Jeyaraman

doi:10.6026/97320630017705

MTT assay based inhibition analysis of A2780 cells proliferation using Mollugo nudicaulis Lam. extract with CXCR4 and HER2 expression

Bioinformation. 2021 Jul 31;17(7):705-709. doi: 10.6026/97320630017705. eCollection 2021.

Authors

Chella Perumal Palanisamy¹, Shazia Fathima Jh², Ramya Sekar³, Ponnulakshmi Rajagopal⁴, Selvaraj Jeyaraman⁵

Affiliations

¹ State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, China.
² Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospitals, Chennai, India.
³ Department of Oral Pathology, Meenakshi Ammal Dental College, Chennai-600 095, India.
⁴ Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai-600 078, India.
⁵ Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai - 600 077, India.

Abstract

It is of interest to document the inhibition of A2780 cell proliferation using Mollugo nudicaulis Lam.(M.nudicaulis) extract by MTT assay and by monitoring the CXCR4 and HER2 expression through RT-PCR analysis. Results shown that the n-hexane extract of M.nudicaulis have anticancer activity IC₅₀ values of 32.46±0.92 µg/mL on A2780 cell lines. It is further found that the CXCR4 and HER2 mRNA and protein expression were significantly reduced in M.nudicaulis treated A2780 cell lines. Thus, the n-hexane extract of M.nudicaulis is a natural source of bioactive compounds as potential anticancer agents.

Keywords: Anti-ovarian cancer activity; CXCR4 and HER2 protein expression; M. nudicaulis; Metastatic signalling; n-hexane extract.