Synergistic action of Viteselen with anti- Fasciola drug as a tool for improving fertility and hemato-biochemical biomarkers in Fasciola infected sheep

Fasciolosis causes public health problems and economic losses all over the world. The present study aimed to evaluate the synergistic action between Viteselen (anti-oxidant) and specific anti-Fasciola drug [Triclalbendazole (TCBZ)] for improving the body condition of F. gigantica naturally infected sheep with reference to some hematological and biochemical biomarkers in their sera. Animals were divided into five groups include G-1 as control non-infected animals, G-2 to G-5 are Fasciola naturally infected animals. G-2 are non-treated animals, G-3 treated with TCBZ, animals in G-4 were injected by Viteselen and those in G-5 were treated by both TCBZ and Viteselen. The results revealed a significant decrease in mean eggs in feces and F. gigantica circulating antigens (FCAg) in sera of TCBZ treated sheep after the 1st week post treatment. Complete disappearance of eggs from feces of drug treated groups was recorded at 21st d.p.t. While the value of FCAg decreased to negative at 14th d.p.t. The highest significant improvement (p < 0.05) in the estimated hematological parameters (RBCs, Hb and TLC), liver enzymes (AST and ALT), oxidative stress and anti-oxidant markers (TAC, MDA, SOD and GSH) and reproductive hormones (Progesterone and Estradiol) was recorded in animals in G-5 followed by G-3. While non-significant improvement was recorded in animals in G-4 in comparison with those in the control group. This improvement increased with increasing the time post treatment. In conclusion; using of Viteselen in association with specific anti-parasitic drug improved the general health parameters and reproductive performance of the investigated sheep.