The direct application of plasma droplet digital PCR in the ultra-early pathogen detection and warning during sepsis: Case reports

J Infect Public Health. 2022 Apr;15(4):450-454. doi: 10.1016/j.jiph.2022.03.008. Epub 2022 Mar 17.

Abstract

Background: Sepsis is a severe and acute disease associated with high mortality, for those who survive, long-term morbidity. In-time detection of pathogen for proper and optimized treatment in the early stage of sepsis is crucial.

Methods: We performed droplet digital PCR (ddPCR) on two cases of sepsis. The clinical information, laboratory test results, and the therapeutic regimen was detailed recorded.

Results: ddPCR showed its clinical value of sepsis ultra-early diagnosis and the guidance of medication in both cases we provided.

Conclusions: ddPCR has potential for rapid and precise diagnose in ultra-early stage of sepsis for its high sensitivity and short turn-around-time, thus benefiting in optimal and timely treatment of sepsis.

Keywords: Droplet digital PCR; Medication guidance; Sepsis; Ultra-early Diagnosis.

Publication types

  • Case Reports

MeSH terms

  • Early Diagnosis
  • Humans
  • Polymerase Chain Reaction / methods
  • Sepsis* / diagnosis