Tuning T cell receptor sensitivity through catch bond engineering

Science. 2022 Apr 8;376(6589):eabl5282. doi: 10.1126/science.abl5282. Epub 2022 Apr 8.

Abstract

Adoptive cell therapy using engineered T cell receptors (TCRs) is a promising approach for targeting cancer antigens, but tumor-reactive TCRs are often weakly responsive to their target ligands, peptide-major histocompatibility complexes (pMHCs). Affinity-matured TCRs can enhance the efficacy of TCR-T cell therapy but can also cross-react with off-target antigens, resulting in organ immunopathology. We developed an alternative strategy to isolate TCR mutants that exhibited high activation signals coupled with low-affinity pMHC binding through the acquisition of catch bonds. Engineered analogs of a tumor antigen MAGE-A3-specific TCR maintained physiological affinities while exhibiting enhanced target killing potency and undetectable cross-reactivity, compared with a high-affinity clinically tested TCR that exhibited lethal cross-reactivity with a cardiac antigen. Catch bond engineering is a biophysically based strategy to tune high-sensitivity TCRs for T cell therapy with reduced potential for adverse cross-reactivity.

MeSH terms

  • Antigens, Neoplasm
  • Cross Reactions
  • Immunotherapy, Adoptive*
  • Major Histocompatibility Complex
  • Myocardium / immunology
  • Peptides
  • Receptors, Antigen, T-Cell*
  • T-Lymphocytes* / metabolism

Substances

  • Antigens, Neoplasm
  • Peptides
  • Receptors, Antigen, T-Cell