Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic

Yi-Jyun Chen; Ying-Pei Hsu; You-Lin Tain; Nan-Si Li; Hao-Han Pang; Shiao-Wei Kuo; Hung-Wei Yang

doi:10.1016/j.bios.2022.114234

Microneedle patches integrated with lateral flow cassettes for blood-free chronic kidney disease point-of-care testing during a pandemic

Biosens Bioelectron. 2022 Jul 15:208:114234. doi: 10.1016/j.bios.2022.114234. Epub 2022 Mar 31.

Authors

Yi-Jyun Chen¹, Ying-Pei Hsu², You-Lin Tain³, Nan-Si Li¹, Hao-Han Pang¹, Shiao-Wei Kuo⁴, Hung-Wei Yang⁵

Affiliations

¹ Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan.
² Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan; Department of Materials and Optoelectronic Science, Center for Functional Polymers and Supramolecular Materials, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
³ Department of Pediatrics, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan; Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 83301, Taiwan.
⁴ Department of Materials and Optoelectronic Science, Center for Functional Polymers and Supramolecular Materials, National Sun Yat-sen University, Kaohsiung 80424, Taiwan; Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Electronic address: [email protected].
⁵ Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan. Electronic address: [email protected].

PMID: 35397328
DOI: 10.1016/j.bios.2022.114234

Abstract

Chronic kidney disease (CKD) is the most neglected chronic disease affecting over 750 million persons in the world. Currently, many patients with cancers or other chronic diseases (i.e., CKD) struggle to receive clinical treatment or examination due to hospitals cancelling or delaying in the COVID-19 pandemic, which may increase the risk of death. Cystatin C (Cys C) has been proposed as a potential glomerular filtration rate (GFR) marker for the early detection of acute kidney injury and CKD. However, most traditional methods for Cys C detection are immunoassays using serum as a sample and are tedious to perform and economically burdensome. To diagnose the disease in the early stage and carry out daily management during the current pandemic, we developed an integration of hydrogel microneedle patch (HMNP) and lateral flow cassette (LFC) to rapidly detect Cys C in skin interstitial fluid (ISF) in 25 min for blood-free CKD management anytime and anywhere by the naked eye that can reduce the impact of an individual's quality of life and life expectancy. Conceivably, this strategy presents a wide scope in the application of numerous other diseases if corresponding analytes are available in skin ISF.

Keywords: Chronic kidney disease; Cystatin C; Interstitial fluid; Lateral flow immunosensor; Microneedles.

MeSH terms

Biosensing Techniques*
COVID-19* / diagnosis
Creatinine
Female
Humans
Male
Pandemics
Point-of-Care Testing
Quality of Life
Renal Insufficiency, Chronic* / diagnosis

Substances

Creatinine