miR-3184-3p enriched in cerebrospinal fluid exosomes contributes to progression of glioma and promotes M2-like macrophage polarization

Cancer Sci. 2022 Aug;113(8):2668-2680. doi: 10.1111/cas.15372. Epub 2022 May 4.

Abstract

Liquid biopsy is a novel strategy for tumour diagnosis. The contents of cerebrospinal fluid (CSF) exosomes could reflect glioma status, hence sampling exosomes from CSF is a means of liquid biopsy for glioma. However, few studies have focused on the function of microRNAs in CSF exosomes. In this study, we found that miR-3184-3p was enriched in CSF exosomes in glioma patients and was downregulated after tumour resection. We found that miR-3184 facilitates glioma progression in two ways. On the one hand, miR-3184 directly promotes proliferation, migration, and invasion while inhibiting apoptosis in glioma. On the other hand, miR-3184 in glioma-derived exosomes polarizes macrophages to an M2-like phenotype, which further aggravates tumour progression. Overall, the current findings uncovered a new mechanism and highlighted the significant role of miR-3184 in glioma progression. Furthermore, exosomal miR-3184 could be a considerable factor with potential applications in glioma diagnosis and treatment in the future.

Keywords: exosomes; gliomas; macrophage; microRNAs; tumor immune microenvironment.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Exosomes* / genetics
  • Exosomes* / pathology
  • Gene Expression Regulation, Neoplastic
  • Glioma* / pathology
  • Humans
  • Macrophages* / pathology
  • MicroRNAs* / cerebrospinal fluid
  • MicroRNAs* / genetics

Substances

  • MIRN3184 microRNA, human
  • MicroRNAs