Characterization of phosphomannan-protein complexes isolated from viable cells of yeast and mycelial forms of Candida albicans NIH B-792 strain by the action of Zymolyase-100T

Arch Biochem Biophys. 1986 Dec;251(2):697-708. doi: 10.1016/0003-9861(86)90379-6.

Abstract

The isolation of phosphomannan-protein complexes from the viable cells of yeast (Y) and mycelial (M) forms of Candida albicans NIH B-792 strain was conducted by treatment with Zymolyase-100T followed by fractional precipitation with cetyltrimethylammonium bromide. The M-form complex was found to contain smaller amount of phosphate (1.3%) than that of the Y-form complex (1.6%). Proton magnetic resonance (PMR) spectra of these complexes indicated that the content of beta-1,2-linked oligomannosyl and nonreducing terminal alpha-1,3-linked mannopyranosyl residues in the M-form complex was lower than that of the Y-form complex. With hot 10 mM HCl, the Y-form complex released a mixture of oligosaccharides ranging from mannose to mannoheptaose, while the M-form complex produced lower oligosaccharides, from mannose to mannotetraose. Upon acetolysis, the acid-modified complex of the M form gave mainly mannotetraose, while that of the Y form produced mainly mannopentaose and mannohexaose in addition to mannotetraose. The average length of branching moieties of the mannan of Y-form cells was therefore longer than that of M-form cells. These results indicate that the Y to M transformation of this C. albicans strain accompanies the suppression of enzyme activity concerning the biosynthesis of mannan such as beta-1,2- and alpha-1,3-mannosyltransferases to synthesize the phosphomannan-protein complex containing mannan moiety with incomplete structure.

MeSH terms

  • Candida albicans / analysis*
  • Candida albicans / growth & development
  • Chromatography, Gel
  • Fungal Proteins / isolation & purification*
  • Hydrolases
  • Magnetic Resonance Spectroscopy
  • Mannans / isolation & purification*
  • Oligosaccharides / analysis
  • Precipitin Tests
  • Protein Binding

Substances

  • Fungal Proteins
  • Mannans
  • Oligosaccharides
  • zymolyase
  • phosphomannan
  • Hydrolases