Abstract
Fatty acids salts exhibit bacteriostatic and bactericidal effects to inhibit bacterial growth and survival. Bacteria adapt to their environment to overcome these antibacterial effects through undefined mechanisms. In Gram-negative bacteria, drug efflux systems are associated with resistance to various substances. Studies have identified multiple drug efflux systems in Salmonella enterica. The aim of this study was to investigate whether drug efflux systems contribute to fatty acid salts resistance in S. enterica. We used deletion and overexpressing strains of S. enterica for drug efflux transporters. Susceptibility to fatty acid salts was determined by measuring minimum inhibitory concentrations and performing growth assays. Our findings revealed that acrAB, acrEF, emrAB and tolC in S. enterica contribute resistance to fatty acid salts. Furthermore, EmrAB, which is known to function with TolC, contributes to the fatty acid salts resistance of S. enterica in a TolC-independent manner. This study revealed that drug efflux systems confer fatty acid satls resistance to S. enterica. Notably, although EmrAB is normally associated with antimicrobial resistance in a TolC-dependent manner, it was found to be involved in fatty acid salts resistance in a TolC-independent manner, indicating that the utilization of TolC by EmrAB is substrate dependent in S. enterica.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / metabolism
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Drug Resistance, Multiple, Bacterial
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Fatty Acids / pharmacology
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Membrane Transport Proteins / genetics
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Microbial Sensitivity Tests
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Salmonella enterica* / genetics
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Salmonella enterica* / metabolism
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Salmonella typhimurium
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Salts* / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Fatty Acids
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Membrane Transport Proteins
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Salts
Grants and funding
This study was supported by a research grant from the Takeda Science Foundation, International Joint Research Promotion Program of Osaka University, Grants-in-Aid for Challenging Research (Exploratory) (18K19451), for Scientific Research (B) (21H03542), for Early-Career Scientists (21K16318) from the Japan Society for the Promotion of Science (JSPS), the Centre of Innovation Program (COI), Core Research for Evolutional Science and Technology (CREST) (JPMJCR20H9) from the Japan Science and Technology Agency (JST), Research Program for CORE laboratory, Network Joint Research Centre for Materials and Devices and Dynamic Alliance for Open Innovation Bridging Human, Environment and Materials from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.