Vlasoulides A and B, a pair of neuroprotective C32 dimeric sesquiterpenes with a hexacyclic 5/7/5/5/(5)/7 carbon skeleton from the roots of Vladimiria souliei

Zhi-Li Wu; Qun Wang; Lu Fu; Ri-Xin Su; Ze-Shi Sun; Hui-Liang Li

doi:10.1039/d1ra00075f

Vlasoulides A and B, a pair of neuroprotective C₃₂ dimeric sesquiterpenes with a hexacyclic 5/7/5/5/(5)/7 carbon skeleton from the roots of Vladimiria souliei

RSC Adv. 2021 Feb 3;11(11):6159-6162. doi: 10.1039/d1ra00075f. eCollection 2021 Feb 2.

Authors

Zhi-Li Wu^{1

2}, Qun Wang², Lu Fu², Ri-Xin Su², Ze-Shi Sun², Hui-Liang Li^{1

2}

Affiliations

¹ School of Traditional Chinese Pharmacy, China Pharmaceutical University Nanjing Jiangsu 211198 P. R. China [email protected].
² School of Pharmacy, Second Military Medical University Shanghai 200433 P. R. China.

Abstract

Vlasoulides A and B (1 and 2), a pair of epimeric C₃₂ sesquiterpene lactone dimers, featuring a 5/7/5/5/(5)/7 ring system were isolated from the roots of Vladimiria souliei. Their chemical structures were determined by comprehensive analysis of spectroscopic data, including HRESIMS and 1D and 2D NMR spectroscopic data. Their absolute configurations were established by Mosher's method and ECD experiments. Furthermore, biological studies showed that compound 1 showed prominent neuroprotective effects against glutamate-induced neurotoxicity in PC-12 cells, with EC₅₀ values of 13.54 ± 0.33 μM, while, the EC₅₀ value of compound 2 is greater than 30 μM.