A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)3Phen) photo probe

Mohmed S Attia; Ahmed O Youssef; Nour A Abdel-Sattar; Mohammed A Amin; Sarah Alharthi; Ekram H Mohamed; Safwat A Mahmoud; Mona N Abou-Omar

doi:10.1039/d1ra07351f

A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)₃Phen) photo probe

RSC Adv. 2022 Feb 3;12(8):4536-4542. doi: 10.1039/d1ra07351f.

Authors

Mohmed S Attia¹, Ahmed O Youssef¹, Nour A Abdel-Sattar¹, Mohammed A Amin², Sarah Alharthi², Ekram H Mohamed³, Safwat A Mahmoud⁴, Mona N Abou-Omar⁵

Affiliations

¹ Chemistry Department, Faculty of Science, Ain Shams University Cairo 11566 Egypt [email protected].
² Department of Chemistry, College of Science, Taif University P.O. Box 11099 Taif 21944 Saudi Arabia.
³ Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt El Sherouk City Cairo Egypt.
⁴ Physics Department, Faculty of Science, Northern Border University Arar Saudi Arabia [email protected].
⁵ Department of Chemistry, Faculty of Women for Arts, Science and Education, Ain Shams University Cairo Egypt [email protected].

Abstract

A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)₃Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)₃Phen) complex in DMSO at pH = 9 and λ _em = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)₃Phen) complex particularly the electrical emission band at λ _em = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis.