This research investigates the use of propionate-cultured sludge to enhance methane (CH4) production and micropollutant biodegradation in biochemical methane potential (BMP) experiment treating landfill leachate. The experiments were carried out using non-acclimatized and acclimatized seed sludge with variable food to microorganism ratios of 1:1 and 1:2. Under the propionate-cultured sludge bioaugmentation, the concentrations of propionate-cultured sludge were varied between 10, 20, and 30 % (v/v). The acclimatized seed sludge exhibited high microbial abundance and diversity which promoted the CH4 production and micropollutant biodegradation. The modified Gompertz model indicated that the optimal condition was the acclimatized seed sludge with 30% (v/v) propionate-cultured sludge, achieving the lag time (λ), maximum CH4 production rate (Rmax), and maximum CH4 potential yield (Pmax) of 0.57 day, 17.35 NmL/h, and 140.58 NmL/g COD. The research novelty lies in the use of propionate-cultured sludge bioaugmentation in landfill leachate treatment to enhance CH4 production and micropollutant biodegradation.
Keywords: Acclimatized seed sludge; Anaerobic digestion; Biochemical methane potential; VFA-degrading bacteria.
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