[Intergeneric conjugational crossing of Escherichia coli with Salmonella typhimurium. II. Transfer of a polA1 mutation from Escherichia coli to Salmonella typhimurium and its phenotypic expression in the salmonella genome]

Genetika. 1977;13(12):2189-94.
[Article in Russian]

Abstract

The Escherichia coli structural gene for DNA polymerase I was inserted into Salmonella typhimurium chromosome by conjugal transfer. The genetic analysis of P1-mediated transduction of obtained hybrid showed that polA gene is located in it between metE and rha loci and is cotransduced with metE (about 50%) and rha (12%). The phenotypic properties of polA1 hybrid E. coliXS. typhimurium concerning UV-MMS-NG and gamma-ray sensitivity are similar to the polA1 mutants of E. coli.

Publication types

  • Comparative Study

MeSH terms

  • Conjugation, Genetic*
  • DNA Polymerase I / genetics*
  • DNA-Directed DNA Polymerase / genetics*
  • Escherichia coli / genetics*
  • Genotype
  • Hybridization, Genetic
  • Mutation
  • Phenotype
  • Salmonella typhimurium / genetics*
  • Transduction, Genetic

Substances

  • DNA Polymerase I
  • DNA-Directed DNA Polymerase