Abstract
In this report, we study the reovirus serotype 3 (strain Dearing) sigma 1 protein obtained from various sources: from Escherichia coli expressing sigma 1 protein, from reovirus-infected mouse L cells, and from purified reovirions. We demonstrate that the sigma 1 protein is a multimer in its undisrupted form and present biochemical evidence suggesting that the multimer is made up of four sigma 1 subunits.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Capsid Proteins*
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Escherichia coli / metabolism
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L Cells / metabolism
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Mammalian orthoreovirus 3 / analysis*
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Mice
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Protein Conformation
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Recombinant Proteins / analysis*
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Reoviridae / analysis*
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Viral Proteins / analysis*
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Virion / analysis
Substances
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Capsid Proteins
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Recombinant Proteins
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Viral Proteins
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sigma 1 protein, reovirus