Facile, Rapid, and Low-Cost Detection for Influenza Viruses and Respiratory Syncytial Virus Based on a Catalytic DNA Assembly Circuit

Huina Wu; Mingyuan Zou; Xiaobo Fan; Feiya Su; Feng Xiao; Meiling Zhou; Yan Sun; Fengfeng Zhao; Guoqiu Wu

doi:10.1021/acsomega.2c00882

Facile, Rapid, and Low-Cost Detection for Influenza Viruses and Respiratory Syncytial Virus Based on a Catalytic DNA Assembly Circuit

ACS Omega. 2022 Apr 19;7(17):15074-15081. doi: 10.1021/acsomega.2c00882. eCollection 2022 May 3.

Authors

Huina Wu¹, Mingyuan Zou¹, Xiaobo Fan¹, Feiya Su¹, Feng Xiao¹, Meiling Zhou¹, Yan Sun¹, Fengfeng Zhao¹, Guoqiu Wu^{2

3

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Affiliations

¹ Medical School of Southeast University, Nanjing 210009, People's Republic of China.
² Center of Clinical Laboratory Medicine, Zhongda Hospital, Southeast University, Nanjing 210009, People's Republic of China.
³ Diagnostics Department, Medical School of Southeast University, Nanjing 210009, People's Republic of China.
⁴ Jiangsu Provincial Key Laboratory of Critical Care Medicine, Southeast University, Nanjing 210009, People's Republic of China.

Abstract

Influenza viruses and respiratory syncytial virus (RSV) have contributed to severe respiratory infections, causing huge economic and healthcare burdens. To achieve rapid and precise detection of influenza viruses and RSV, we proposed a catalytic hairpin assembly (CHA) combined with the lateral flow immunoassay (CHA-LFIA) detection method. The presence of the target RNA triggers the initiation of CHA circuits. H1/H2 complexes, the amplified signal products, which were labeled with digoxin and biotin, were detected with a highly sensitive lateral flow immunoassay system. The sensitivity of the CHA-LFIA system to influenza A and B viruses and RSV reached up to 1, 1, and 5 pM, respectively. In addition, this method exhibited excellent capability for differentiating between target RNA and base-mismatched RNA. The results demonstrated that an enzyme-free, rapid, highly sensitive, and specific method had been developed to detect influenza A and B viruses and RSV.