Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model

Yumei Li; Jiyoung Lee; Doumet Georges Helou; Omid Akbari; Jing-Hsiung James Ou

doi:10.1016/j.xpro.2022.101364

Analysis of the interplay between hepatitis B virus-positive hepatocytes and Kupffer cells ex vivo using mice as a model

STAR Protoc. 2022 May 6;3(2):101364. doi: 10.1016/j.xpro.2022.101364. eCollection 2022 Jun 17.

Authors

Yumei Li¹, Jiyoung Lee¹, Doumet Georges Helou¹, Omid Akbari¹, Jing-Hsiung James Ou¹

Affiliation

¹ Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, Los Angeles, CA 90033, USA.

Abstract

Kupffer cells play critical roles in both hepatitis B virus (HBV) persistence and clearance. Here, we provide a protocol for studying the interplay between Kupffer cells and HBV-positive hepatocytes ex vivo using mice as a model. This protocol includes hydrodynamic injection of HBV DNA into mouse hepatocytes, liver perfusion for isolating hepatocytes and Kupffer cells, and Seahorse metabolic analysis of Kupffer cells. This protocol allows the detailed analysis of how HBV-positive hepatocytes and Kupffer cells impact each other ex vivo. For complete details on the use and execution of this protocol, please refer to Li et al. (2022).

Keywords: Cell Biology; Cell culture; Cell isolation; Immunology; Metabolism; Microbiology; Model Organisms.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Hepatitis B virus* / genetics
Hepatocytes
Kupffer Cells*
Liver
Mice
Perfusion

Abstract

Publication types

MeSH terms

Grants and funding