[Down-regulation of tripartite motif containing 59 (TRIM59) blocks the NF-κB signaling pathway and inhibits the invasion and migration of nasopharyngeal carcinoma cells]

Objective To investigate whether tripartite motif containing 59 (TRIM59) influences the biological behavior of nasopharyngeal carcinoma cells by regulating the nuclear factor κB(NF-κB) signaling pathway. Methods TCGA database was used to predict the expression of TRIM59 in nasopharyngeal carcinoma and adjacent tissues. Reverse transcription PCR and Western blot were used to detect the relative expressions of TRIM59 mRNA and protein in nasopharyngeal carcinoma and paracancerous tissues. With human normal nasal mucosal epithelial cells (HNEpCs) as a control, reverse transcription PCR and Western blot analysis were used to detect the relative expression of TRIM59 mRNA and protein in HNE1 and CNE-2Z nasopharyngeal carcinoma cells. Small interference RNA technology was used to down-regulate the level of TRIM59 in HNE1 cells, while a control group, small interference RNA negative control (si-NC) group and TRIM59 small interference RNA (si-TRIM59) group were set up. MTT assay was used to detect the cell proliferation inhibition rate of each transfection group. Transwell^TM chamber was used to detect cell invasion and migration ability of each transfection group. Western blot analysis was employed to detect NF-κB and phosphorylated NF-κB (p-NF-κB), vimentin, survivin protein expression. ELISA was adopted to detect interleukin 6 (IL-6), IL-8, tumor necrosis factor α (TNF-α) in the supernatant of cultured cells. Results The expression of TRIM59 increased abnormally in nasopharyngeal carcinoma tissues. Compared with HNEpC cells, the relative expression of TRIM59 in HNE1 significantly increased. After down-regulating the expression of TRIM59 in HNE1 cells, cell survival and cell invasion and migration were significantly inhibited. Down-regulating the expression of TRIM59 inhibited HNE1 cells p-NF-κB, vimentin, survivin protein expression and significantly reduced the levels of IL-6, IL-8, and TNF-α. Conclusion Down-regulation of TRIM59 blocks the NF-κB signaling pathway and tumor inflammation-related factors in HNE1 cells, thereby inhibiting cell invasion and migration.