Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction

Honorine Fenaux; Lamia Limam; Marie-Pierre Soutiere; Frédéric Veillet; Vanessa Escuret; Anne-Marie Roque-Afonso

doi:10.1016/j.diagmicrobio.2022.115700

Performance of the QIAprep&amp Viral RNA UM Kit assay (Qiagen), an automatable method for RT-qPCR detection of SARS-CoV-2 without RNA extraction

Diagn Microbiol Infect Dis. 2022 Jul;103(3):115700. doi: 10.1016/j.diagmicrobio.2022.115700. Epub 2022 Apr 21.

Authors

Honorine Fenaux¹, Lamia Limam¹, Marie-Pierre Soutiere¹, Frédéric Veillet¹, Vanessa Escuret², Anne-Marie Roque-Afonso³

Affiliations

¹ Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France.
² Hospices Civils de Lyon, Laboratoire associé au Centre National de Référence des virus des infections respiratoires, IAI, CBN, Lyon Cedex04, France; Univ Lyon, Université Claude Bernard Lyon 1, ENS Lyon, CIRI, Inserm U1111, CNRS UMR5308, Lyon, France.
³ Laboratoire de virologie, hôpital Paul Brousse, Assistance Publique, Hôpitaux de Paris, France. Electronic address: [email protected].

Abstract

We evaluated the performance of the QIAprep&amp Viral RNA UM Kit (Qiagen) for SARS-CoV-2 detection. It displayed specificity and sensitivity required for SARS-CoV-2 RNA detection from swab transport media without RNA extraction. This method identifies accurately patients at risk of transmission while saving time and cost of extraction.

Keywords: RT-qPCR; SARS-CoV-2; cellular control; low volumes; rapid diagnosis.

MeSH terms

COVID-19 Nucleic Acid Testing
COVID-19* / diagnosis
Humans
RNA, Viral / analysis
RNA, Viral / genetics
SARS-CoV-2* / genetics
Sensitivity and Specificity

Substances

RNA, Viral