N-Terminomics/TAILS of Tissue and Liquid Biopsies

Anthonia Anowai; Sameeksha Chopra; Barbara Mainoli; Daniel Young; Antoine Dufour

doi:10.1007/978-1-0716-2124-0_7

N-Terminomics/TAILS of Tissue and Liquid Biopsies

Methods Mol Biol. 2022:2456:85-94. doi: 10.1007/978-1-0716-2124-0_7.

Authors

Anthonia Anowai^{1

2

3

4}, Sameeksha Chopra^{1

2

3

4}, Barbara Mainoli^{1

2

3

4}, Daniel Young^{1

2

3

4}, Antoine Dufour^{5

6

7

8}

Affiliations

¹ Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.
² Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada.
³ McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada.
⁴ Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada.
⁵ Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada. [email protected].
⁶ Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada. [email protected].
⁷ McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada. [email protected].
⁸ Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada. [email protected].

PMID: 35612737
DOI: 10.1007/978-1-0716-2124-0_7

Abstract

The N-terminomics approach of Terminal Amine Isotopic Labeling of Substrates (TAILS) enables the identification and quantification of natural and neo-N-termini of proteins using liquid chromatography and tandem mass spectrometry (LC-MS/MS). This methodology has been used to study protease function and identify protease substrates in cell culture systems, animal disease models, and more recently, has been applied to clinical samples. Here, we present the application of TAILS to tissue and liquid biopsies.

Keywords: Biopsy; Mass spectrometry; N-terminomics; Protease; Proteomics; TAILS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amines* / chemistry
Animals
Chromatography, Liquid
Isotope Labeling / methods
Liquid Biopsy
Peptide Hydrolases / metabolism
Proteome / metabolism
Proteomics* / methods
Tandem Mass Spectrometry / methods

Substances

Amines
Proteome
Peptide Hydrolases