Phosphoproteomics and Organelle Proteomics in Pancreatic Islets

Özüm Sehnaz Caliskan; Giorgia Massacci; Natalie Krahmer; Francesca Sacco

doi:10.1007/978-1-0716-2124-0_9

Phosphoproteomics and Organelle Proteomics in Pancreatic Islets

Methods Mol Biol. 2022:2456:123-140. doi: 10.1007/978-1-0716-2124-0_9.

Authors

Özüm Sehnaz Caliskan^#^{1

2}, Giorgia Massacci^#³, Natalie Krahmer^{4

5}, Francesca Sacco⁶

Affiliations

¹ Diabetes Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.
² German Center for Diabetes Research (DZD), Neuherberg, Germany.
³ Department of Biology, University of Rome Tor Vergata, Rome, Italy.
⁴ Diabetes Center, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany. [email protected].
⁵ German Center for Diabetes Research (DZD), Neuherberg, Germany. [email protected].
⁶ Department of Biology, University of Rome Tor Vergata, Rome, Italy. [email protected].

^# Contributed equally.

PMID: 35612739
DOI: 10.1007/978-1-0716-2124-0_9

Abstract

Over the recent years, mass spectrometry (MS)-based proteomics has undergone dramatic advances in sample preparation, instrumentation, and computational methods. Here, we describe in detail, how a workflow quantifies global protein phosphorylation in pancreatic islets and characterizes intracellular organelle composition on protein level by MS-based proteomics.

Keywords: Mass spectrometry-based phosphoproteomics; Organelles; Pancreatic islets; Protein correlation profiling.

MeSH terms

Islets of Langerhans* / metabolism
Mass Spectrometry / methods
Organelles / metabolism
Phosphorylation
Proteomics* / methods