Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

Dian Kortleve; Mandy van Brakel; Rebecca Wijers; Reno Debets; Dora Hammerl

doi:10.1007/978-1-0716-2115-8_13

Gene Engineering T Cells with T-Cell Receptor for Adoptive Therapy

Methods Mol Biol. 2022:2453:209-229. doi: 10.1007/978-1-0716-2115-8_13.

Authors

Dian Kortleve¹, Mandy van Brakel², Rebecca Wijers², Reno Debets², Dora Hammerl²

Affiliations

¹ Laboratory of Tumor Immunology, Department of Medical Oncology, Erasmus MC-Cancer Institute, Rotterdam, The Netherlands. [email protected].
² Laboratory of Tumor Immunology, Department of Medical Oncology, Erasmus MC-Cancer Institute, Rotterdam, The Netherlands.

Abstract

Prior to clinical testing of adoptive T-cell therapy with T-cell receptor (TCR)-engineered T cells, TCRs need to be retrieved, annotated, gene-transferred, and extensively tested in vitro to accurately assess specificity and sensitivity of target recognition. Here, we present a fundamental series of protocols that cover critical preclinical parameters, thereby enabling the selection of candidate TCRs for clinical testing.

Keywords: Gene transfer; In vitro assays; Sensitivity; Specificity; T-cell engineering; T-cell receptor; TCR annotation; TCR cloning.

MeSH terms

Genetic Engineering
Immunotherapy, Adoptive / methods
Lymphocyte Count
Receptors, Antigen, T-Cell* / genetics
T-Lymphocytes*

Substances

Receptors, Antigen, T-Cell