Normative values for esophageal functional lumen imaging probe measurements: A meta-analysis

Background: The functional lumen imaging probe (Endoflip™) is increasingly used for evaluation of patients with esophageal symptoms. To improve the interpretation of Endoflip™ in clinical practice, normative values with appropriate cut-off values are required.

Methods: Original clinical studies describing Endoflip™ use for measurements of esophageal motility in healthy adults were considered. Meta-analysis was performed based on published values.

Results: A total of 17 articles were included in the systematic review, 15 of which were included in the meta-analysis, representing 154 unique subjects. At 40 ml distention, the 5th-95th and 10th-90th percentiles for esophagogastric junction distensibility index (EGJ-DI) were 1.96-10.95 mm² /mmHg and 2.36-8.95 mm² /mmHg, respectively. An EGJ-DI below 2 mm² /mmHg was found in 5.4%, and below 3 mm² /mmHg in 20.1% of healthy subjects. At 50 ml distention, the 5th-95th and 10th-90th percentiles for EGJ-DI are 2.86-10.66 mm² /mmHg and 3.28-9.12 mm² /mmHg, respectively (below 2 mm² /mmHg: 0.6%, 3 mm² /mmHg: 6.3%). The 5th-95th and 10th-90th percentiles for EGJ-DI at 60 ml distention were 3.06-8.14 mm² /mmHg and 3.33-7.18 mm² /mmHg, respectively (below 2 mm² /mmHg: 0.0%, 3 mm² /mmHg: 7%). A clear cut-off for lower values was identified while a large spread in values was observed for upper limits of normal for EGJ-DI for all filling volumes.

Conclusions: Given these observations, we recommend using a cut-off of 2 mm² /mmHg for clinical practice, values below can be considered abnormal. Given that 5.4% of the healthy subjects will have an EGJ-DI below 2 mm² /mmHg at 40 ml, we recommend using the 50 and 60 ml distention volumes. The clinical use of an upper limit for normality of EGJ-DI seems questionable.