Rapid and sensitive multiplex detection of COVID-19 antigens and antibody using electrochemical immunosensor-/aptasensor-enabled biochips

Fuze Jiang; Zhen Xiao; Ting Wang; Jiajia Wang; Lihua Bie; Lanja Saleh; Kathrin Frey; Lianjun Zhang; Jing Wang

doi:10.1039/d2cc01598f

Rapid and sensitive multiplex detection of COVID-19 antigens and antibody using electrochemical immunosensor-/aptasensor-enabled biochips

Chem Commun (Camb). 2022 Jun 28;58(52):7285-7288. doi: 10.1039/d2cc01598f.

Authors

Fuze Jiang^{1

2}, Zhen Xiao^{3

4}, Ting Wang⁵, Jiajia Wang^{3

4

6}, Lihua Bie⁷, Lanja Saleh⁸, Kathrin Frey⁸, Lianjun Zhang^{3

4}, Jing Wang^{1

2}

Affiliations

¹ Institute of Environmental Engineering, ETH Zürich, Zürich, Switzerland. [email protected].
² Advanced Analytical Technologies, Empa, Dübendorf, Switzerland.
³ CAMS Key Laboratory of Synthetic Biology Regulatory Element, Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. [email protected].
⁴ Suzhou Institute of Systems Medicine, Suzhou 215123, China.
⁵ School of Automation, Hangzhou Dianzi University, Hangzhou 310018, China.
⁶ School of Engineering, China Pharmaceutical University, Nanjing, China.
⁷ College of Informatics, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
⁸ Institute of Clinical Chemistry, University Hospital Zurich, University of Zurich, Zurich 8091, Switzerland.

Abstract

We report protein- and aptamer-based electrochemical biochips for low-cost, one-step, sensitive and accurate multiplex detection of SARS-CoV-2 spike (S) and nucleocapsid (N) proteins, and IgG antibody in unprocessed clinical samples, allowing citizens to achieve rapid diagnosis at home or in community settings.

MeSH terms

Antibodies, Viral
Biosensing Techniques*
COVID-19* / diagnosis
Electrochemical Techniques
Humans
Immunoassay
SARS-CoV-2
Spike Glycoprotein, Coronavirus

Substances

Antibodies, Viral
Spike Glycoprotein, Coronavirus
spike protein, SARS-CoV-2