Measuring trogocytosis between ovarian tumor and natural killer cells

Antonio Delgado-Gonzalez; Ying-Wen Huang; Ermelinda Porpiglia; Kenyi Donoso; Veronica D Gonzalez; Wendy J Fantl

doi:10.1016/j.xpro.2022.101425

Measuring trogocytosis between ovarian tumor and natural killer cells

STAR Protoc. 2022 Jun 7;3(2):101425. doi: 10.1016/j.xpro.2022.101425. eCollection 2022 Jun 17.

Authors

Antonio Delgado-Gonzalez^{1

2}, Ying-Wen Huang^{1

2}, Ermelinda Porpiglia^{2

3}, Kenyi Donoso¹, Veronica D Gonzalez², Wendy J Fantl^{1

4

5}

Affiliations

¹ Deparment of Urology, Stanford University School of Medicine, Stanford, CA 94305, USA.
² Baxter Laboratory for Stem Cell Biology, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
³ Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
⁴ Stanford Cancer Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
⁵ Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Abstract

Trogocytosis is an active transport mechanism by which one cell extracts a plasma membrane fragment with embedded molecules from an adjacent cell in a contact-dependent process leading to the acquisition of a new function. Our protocol, which has general applicability, consolidates and optimizes existing protocols while highlighting key experimental variables to demonstrate that natural killer (NK) cells acquire the tetraspanin CD9 by trogocytosis from ovarian tumor cells. For complete details on the use and execution of this protocol, please refer to Gonzalez et al. (2021).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Microscopy; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Cell Membrane / metabolism
Female
Humans
Killer Cells, Natural / metabolism
Ovarian Neoplasms* / metabolism
Trogocytosis*

Abstract

Publication types

MeSH terms

Grants and funding