Detection of exogenous DNA uptake by murine dendritic cells

STAR Protoc. 2022 Jun 14;3(3):101464. doi: 10.1016/j.xpro.2022.101464. eCollection 2022 Sep 16.

Abstract

This protocol has been developed to measure exogenous DNA uptake by murine dendritic cells (DCs) using supernatant containing cellular debris, which allows for DNA uptake in the absence of transfection reagents. Inhibitors or antibodies that alter the process can be added, and either flow cytometry or fluorescent microscopy can be used to measure DNA uptake. This is intended to mimic the exposure of DCs to dying cells in the tumor microenvironment or other pathological conditions of high cellular death. For complete details on the use and execution of this protocol, please refer to de Mingo Pulido et al. (2021).

Keywords: Cancer; Cell Biology; Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Microscopy; Molecular/Chemical Probes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • DNA*
  • Dendritic Cells*
  • Mice

Substances

  • DNA