RuO2/rGO heterostructures as mimic peroxidases for colorimetric detection of glucose

Haolin Li; Huiyuan Sun; Jianwei Ding; Ting Wu; Shuangfei Cai; Chen Wang; Rong Yang

doi:10.1007/s00604-022-05319-0

RuO₂/rGO heterostructures as mimic peroxidases for colorimetric detection of glucose

Mikrochim Acta. 2022 Jun 21;189(7):261. doi: 10.1007/s00604-022-05319-0.

Authors

Haolin Li^{1

2}, Huiyuan Sun¹, Jianwei Ding¹, Ting Wu¹, Shuangfei Cai³, Chen Wang⁴, Rong Yang^{5

6}

Affiliations

¹ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, 100190, China.
² Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing, 100049, China.
³ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, 100190, China. [email protected].
⁴ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, 100190, China. [email protected].
⁵ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, 100190, China. [email protected].
⁶ Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing, 100049, China. [email protected].

PMID: 35727400
DOI: 10.1007/s00604-022-05319-0

Abstract

The successful synthesis of ruthenium oxide/reduced graphene oxide (RuO₂/rGO) heterostructures by one-pot hydrothermal method using graphene oxides and RuCl₃ as precursors is reported. The heterostructures had high peroxidase-like (POD-like) activities, which catalyzes the oxidation of classical peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H₂O₂ to create a blue colored reaction product. The catalytic activity was significantly enhanced by the synergistic effect between RuO₂ nanoparticles and rGO. RuO₂/rGO had a low K_m of 0.068 mM and a high v_max of 1.228 × 10^-7 M·s^-1 towards TMB in the TMB-H₂O₂ catalytic oxidation system. In addition, the POD-like activity originating from the electron transfer mechanism was confirmed by cytochrome C (Cyt C) oxidation experiment. A colorimetric method based on RuO₂/rGO heterostructures was developed with good sensitivity and selectivity for glucose detection with a limit of detection of 3.34 μM and a linear range of 0-1500 μM. The RuO₂/rGO heterostructures have potential applications in the biomedical areas, such as biosensor and diagnostics.

Keywords: Colorimetric detection; Glucose detection; Mimic enzyme; Peroxidase-like activity; RuO2/rGO heterostructures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry* / methods
Glucose
Graphite
Hydrogen Peroxide / chemistry
Peroxidases*

Substances

graphene oxide
Graphite
Hydrogen Peroxide
Peroxidases
Glucose

Abstract

Publication types

MeSH terms

Substances

Grants and funding