Evaluating the neuroprotective activities of vinpocetine, punicalagin, niacin and vitamin E against behavioural and motor disabilities of manganese-induced Parkinson's disease in Sprague Dawley rats

Biomed Pharmacother. 2022 Sep:153:113330. doi: 10.1016/j.biopha.2022.113330. Epub 2022 Jun 30.

Abstract

The current study investigated the neuroprotective activity of some drugs and nutriceuticals with antioxidant and anti-inflammatory potential on the pathogenesis of Parkinson's disease (PD). Rats were categorized into seven groups: Rats received tween80 daily for 5 weeks as a control group, MnCl2 (10 mg/kg, i.p) either alone (group II) or in combination with vinpocetine (VIN) (20 mg/kg) (group III), punicalagin (PUN) (30 mg/kg) (group IV), niacin (85 mg/kg) (group V), vitamin E (Vit E) (100 mg/kg) (group VI) or their combination (group VII). Motor activities was examined using open-field and catalepsy. Striatal monamines, acetylcholinesterase, excitatory/inhibitory neurotransmitters, redox status, pro-oxidant content, brain inflammatory, apoptotic and antioxidant biomarkers levels were assessed. Besides, histopathological investigations of different brain regions were determined. Groups (IV -GVII) showed improved motor functions of PD rats. Applied drugs significantly increased the brain levels of monoamines with the strongest effect to PUN. Meanwhile, they significantly decreased levels of acetylcholinesterase with a strongest effect to PUN. Moreover, they exhibited significant neuronal protection and anti-inflammatory abilities through significant reduction of the brain levels of COX2, TNF-α and Il-1β with a strongest effect to the PUN. Interestingly; groups (IV - GVII) showed restored glutamate/GABA balance and exhibited a pronounced decrease in caspase-3 content and GSK-3β protein expression levels. In addition, they significantly increased Bcl2 mRNA expression levels with a strongest effect for PUN. All these findings were further confirmed by the histopathological examinations. As a conclusion, we propose VIN and PUN to mitigate the progression of PD via their antioxidant, anti-inflammatory, anti-apoptotic, neurotrophic and neurogenic activities.

Keywords: Niacin; Oxidative stress; Parkinson’s disease; Punicalagin; Vinpocetine; Vitamin E.

MeSH terms

  • Acetylcholinesterase
  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use
  • Glycogen Synthase Kinase 3 beta
  • Hydrolyzable Tannins
  • Manganese / pharmacology
  • Neuroprotection
  • Neuroprotective Agents* / pharmacology
  • Neuroprotective Agents* / therapeutic use
  • Niacin* / pharmacology
  • Oxidative Stress
  • Parkinson Disease* / drug therapy
  • Rats
  • Rats, Sprague-Dawley
  • Vinca Alkaloids
  • Vitamin E / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Hydrolyzable Tannins
  • Neuroprotective Agents
  • Vinca Alkaloids
  • Vitamin E
  • Niacin
  • Manganese
  • vinpocetine
  • punicalagin
  • Glycogen Synthase Kinase 3 beta
  • Acetylcholinesterase