Empagliflozin Attenuates Obesity-Related Kidney Dysfunction and NLRP3 Inflammasome Activity Through the HO-1-Adiponectin Axis

Front Endocrinol (Lausanne). 2022 Jun 17:13:907984. doi: 10.3389/fendo.2022.907984. eCollection 2022.

Abstract

Empagliflozin (EMPA) is a novel sodium-glucose cotransporter 2 inhibitor (SGLT2i) that produces protective cardiovascular-renal outcomes in patients with diabetes. However, the effects of EMPA on obesity-related kidney disease have not been determined. The heme oxygenase-1 (HO-1)-adiponectin axis is an essential antioxidant system with anti-apoptotic and anti-inflammatory properties. This study explored whether EMPA improves obesity-related kidney disease through regulation of the renal HO-1-mediated adiponectin axis. C57BL/6J mice were assigned to control, high-fat diet (HFD) groups, and EMPA (10 mg/kg) groups. HFD mice showed metabolic abnormality and renal injury, including increased urinary albumin excretion, morphologic changes, and lipid accumulation. EMPA treatment improved metabolic disorders and attenuated lipotoxicity-induced renal injury. Furthermore, EMPA treatment ameliorated renal NLRP3 inflammasome activity and upregulated the HO-1-adiponectin axis. Our findings indicate that EMPA improves obesity-related kidney disease through reduction of NLRP3 inflammasome activity and upregulation of the HO-1-adiponectin axis, suggesting a novel mechanism for SGLT2i-mediated renal protection in obesity.

Keywords: Empagliflozin; HO-1; NLRP3; kidney disease; obesity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin*
  • Animals
  • Benzhydryl Compounds
  • Glucosides
  • Heme Oxygenase-1
  • Inflammasomes*
  • Kidney
  • Membrane Proteins
  • Mice
  • Mice, Inbred C57BL
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Obesity / complications
  • Obesity / drug therapy

Substances

  • Adiponectin
  • Benzhydryl Compounds
  • Glucosides
  • Inflammasomes
  • Membrane Proteins
  • NLR Family, Pyrin Domain-Containing 3 Protein
  • Nlrp3 protein, mouse
  • Heme Oxygenase-1
  • Hmox1 protein, mouse
  • empagliflozin