Ultrasound measures of muscle thickness and subcutaneous tissue from the hip abductors: Inter- and intra-rater reliability

Background: Ultrasound imaging is important in many fields such as medicine, sports, and health sciences to assess parts of muscle structure (e.g., muscle thickness [MT]) or composition (subcutaneous tissue [Sub_T]).

Objective: The aim of the present study was to investigate the intra- and inter-rater reliability of MT and Sub_T measurements of the hip abductor muscles gluteus medius (GM) and tensor fascia latae (TFL).

Design: Cross-sectional study.

Method: Twenty young adults participated in the study. Intra-rater reliability was established by measuring the same two images twice by the same rater, while inter-rater reliability was assessed between two raters by measuring the same two images for each muscle. For both intra- and inter-rater reliability, the reliability of the TFL and GM outcomes (MT- Sub_T) were determined by the intraclass correlation coefficient (ICC), coefficient of variation (CV), standard error of the measurement (SEM), and Bland-Altman plots.

Results: For intra-rater reliability, variables of both muscles showed an excellent ICC (≥0.90), lower CV and SEM, and bias near zero. Inter-rater reliability also showed an excellent ICC for both variables and muscles (≥0.81) with lower CV, SEM, and bias.

Conclusion: Therefore, these results provide strong evidence of a reliable measure of MT and Sub_T from GM and TFL. The present study provides health care professionals and researchers increased confidence in using 2D ultrasound to assess the hip abductors muscles reliably.