The first total synthesis of ogipeptin A was achieved. Recently, using the advanced Marfey's method, we determined the absolute configuration patterns of three β-hydroxy-α,γ-diaminobutyric acids (β-OH Dabs) composing ogipeptins. On the basis of this result, we conducted solid-phase total synthesis of three diastereomers of ogipeptin A. The analytical data of one diastereomer exactly corresponded with those of natural ogipeptin A. Therefore, the absolute configurations of ogipeptins have been elucidated.