Detecting MicroRNAs in Plant Genomes with miRkwood

Methods Mol Biol. 2022:2512:103-120. doi: 10.1007/978-1-0716-2429-6_8.

Abstract

We present miRkwood, a comprehensive software tool developed to identify microRNAs and their precursor in plant genomes, with or without small-RNA-seq sequencing data. We describe how to install the software, how to set up and run it, and how to explore and analyse the results: genomic annotations, secondary structure of the precursor, alignments, reads distribution.

Keywords: Bioinformatics; High throughput sequencing; MicroRNA; Small RNA sequencing.

MeSH terms

  • Genome, Plant
  • Genomics
  • High-Throughput Nucleotide Sequencing
  • MicroRNAs* / chemistry
  • MicroRNAs* / genetics
  • Sequence Analysis, RNA
  • Software

Substances

  • MicroRNAs