This article details the development of a single multiplex system amplifying 26 rapidly mutating Y-STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y-STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.
Keywords: STRs; discrimination; multiplex; paternal; rapidly mutating.
© 2022 The Authors. Electrophoresis published by Wiley-VCH GmbH.