Phase 2 of extracellular RNA communication consortium charts next-generation approaches for extracellular RNA research

Bogdan Mateescu; Jennifer C Jones; Roger P Alexander; Eric Alsop; Ji Yeong An; Mohammad Asghari; Alex Boomgarden; Laura Bouchareychas; Alfonso Cayota; Hsueh-Chia Chang; Al Charest; Daniel T Chiu; Robert J Coffey; Saumya Das; Peter De Hoff; Andrew deMello; Crislyn D'Souza-Schorey; David Elashoff; Kiarash R Eliato; Jeffrey L Franklin; David J Galas; Mark B Gerstein; Ionita H Ghiran; David B Go; Stephen Gould; Tristan R Grogan; James N Higginbotham; Florian Hladik; Tony Jun Huang; Xiaoye Huo; Elizabeth Hutchins; Dennis K Jeppesen; Tijana Jovanovic-Talisman; Betty Y S Kim; Sung Kim; Kyoung-Mee Kim; Yong Kim; Robert R Kitchen; Vaughan Knouse; Emily L LaPlante; Carlito B Lebrilla; L James Lee; Kathleen M Lennon; Guoping Li; Feng Li; Tieyi Li; Tao Liu; Zirui Liu; Adam L Maddox; Kyle McCarthy; Bessie Meechoovet; Nalin Maniya; Yingchao Meng; Aleksandar Milosavljevic; Byoung-Hoon Min; Amber Morey; Martin Ng; John Nolan; Getulio P De Oliveira Junior; Michael E Paulaitis; Tuan Anh Phu; Robert L Raffai; Eduardo Reátegui; Matthew E Roth; David A Routenberg; Joel Rozowsky; Joseph Rufo; Satyajyoti Senapati; Sigal Shachar; Himani Sharma; Anil K Sood; Stavros Stavrakis; Alessandra Stürchler; Muneesh Tewari; Juan P Tosar; Alexander K Tucker-Schwartz; Andrey Turchinovich; Nedyalka Valkov; Kendall Van Keuren-Jensen; Kasey C Vickers; Lucia Vojtech; Wyatt N Vreeland; Ceming Wang; Kai Wang; ZeYu Wang; Joshua A Welsh; Kenneth W Witwer; David T W Wong; Jianping Xia; Ya-Hong Xie; Kaichun Yang; Mikołaj P Zaborowski; Chenguang Zhang; Qin Zhang; Angela M Zivkovic; Louise C Laurent

doi:10.1016/j.isci.2022.104653

Phase 2 of extracellular RNA communication consortium charts next-generation approaches for extracellular RNA research

iScience. 2022 Jun 23;25(8):104653. doi: 10.1016/j.isci.2022.104653. eCollection 2022 Aug 19.

Authors

Bogdan Mateescu^{1

2}, Jennifer C Jones³, Roger P Alexander⁴, Eric Alsop⁵, Ji Yeong An⁶, Mohammad Asghari², Alex Boomgarden⁷, Laura Bouchareychas^{8

9}, Alfonso Cayota^{10

11}, Hsueh-Chia Chang^{12

13}, Al Charest¹⁴, Daniel T Chiu¹⁵, Robert J Coffey^{16

17}, Saumya Das¹⁸, Peter De Hoff¹⁹, Andrew deMello², Crislyn D'Souza-Schorey⁷, David Elashoff²⁰, Kiarash R Eliato²¹, Jeffrey L Franklin^{16

17}, David J Galas²², Mark B Gerstein^{23

24

25}, Ionita H Ghiran¹⁴, David B Go^{12

13}, Stephen Gould²⁶, Tristan R Grogan²⁷, James N Higginbotham¹⁶, Florian Hladik^{28

29}, Tony Jun Huang³⁰, Xiaoye Huo¹², Elizabeth Hutchins⁵, Dennis K Jeppesen¹⁶, Tijana Jovanovic-Talisman²¹, Betty Y S Kim³¹, Sung Kim⁶, Kyoung-Mee Kim³², Yong Kim³³, Robert R Kitchen³⁴, Vaughan Knouse³, Emily L LaPlante³⁵, Carlito B Lebrilla³⁶, L James Lee³⁷, Kathleen M Lennon²¹, Guoping Li¹⁸, Feng Li³³, Tieyi Li³⁸, Tao Liu³⁹, Zirui Liu³⁸, Adam L Maddox²¹, Kyle McCarthy¹², Bessie Meechoovet⁵, Nalin Maniya¹², Yingchao Meng², Aleksandar Milosavljevic^{35

40}, Byoung-Hoon Min⁴¹, Amber Morey¹⁹, Martin Ng⁹, John Nolan⁴², Getulio P De Oliveira Junior¹⁴, Michael E Paulaitis⁴³, Tuan Anh Phu⁹, Robert L Raffai^{8

9

44}, Eduardo Reátegui³⁷, Matthew E Roth³⁵, David A Routenberg⁴⁵, Joel Rozowsky²³, Joseph Rufo³⁰, Satyajyoti Senapati¹², Sigal Shachar⁴⁵, Himani Sharma¹², Anil K Sood⁴⁶, Stavros Stavrakis², Alessandra Stürchler^{1

2}, Muneesh Tewari^{47

48

49

50}, Juan P Tosar^{10

51}, Alexander K Tucker-Schwartz⁴⁵, Andrey Turchinovich^{52

53}, Nedyalka Valkov¹⁸, Kendall Van Keuren-Jensen⁵, Kasey C Vickers⁵⁴, Lucia Vojtech⁵⁵, Wyatt N Vreeland⁵⁶, Ceming Wang¹², Kai Wang⁵⁷, ZeYu Wang³⁰, Joshua A Welsh³, Kenneth W Witwer^{58

59}, David T W Wong³³, Jianping Xia³⁰, Ya-Hong Xie³⁸, Kaichun Yang³⁰, Mikołaj P Zaborowski⁶⁰, Chenguang Zhang¹², Qin Zhang¹⁶, Angela M Zivkovic⁶¹, Louise C Laurent¹⁹

Affiliations

¹ Brain Research Institute, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
² Institute for Chemical and Bioengineering, ETH Zürich, Vladimir Prelog Weg 1, 8093 Zürich, Switzerland.
³ Laboratory of Pathology Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
⁴ Extracellular RNA Communication Consortium, Phoenix, AZ 85254, USA.
⁵ Neurogenomics Division, TGen, Phoenix, AZ 85004, USA.
⁶ Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
⁷ Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
⁸ Department of Surgery, Division of Vascular and Endovascular Surgery, University of California, San Francisco, San Francisco, CA 94143, USA.
⁹ Northern California Institute for Research and Education, San Francisco, CA 94121, USA.
¹⁰ Functional Genomics Unit, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay.
¹¹ University Hospital, Universidad de la República, Montevideo 11600, Uruguay.
¹² Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
¹³ Department of Aerospace and Mechanical Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
¹⁴ Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
¹⁵ Department of Chemistry and Bioengineering, University of Washington, Seattle, WA 98195, USA.
¹⁶ Department of Medicine/Gastroenterology and Epithelial Biology Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
¹⁷ Cell and Developmental Biology, Vanderbilt University, Nashville, TN 37235, USA.
¹⁸ Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA.
¹⁹ Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, La Jolla, San Diego, CA 92093, USA.
²⁰ Statistics Core, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
²¹ Department of Molecular Medicine, Beckman Research Institute of the City of Hope Comprehensive Cancer Center, Duarte, CA 91010, USA.
²² Pacific Northwest Research Institute, Seattle, WA 98122, USA.
²³ Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA.
²⁴ Program in Computational Biology & Bioinformatics, Yale University, New Haven, CT 06520, USA.
²⁵ Department of Computer Science, Yale University, New Haven, CT 06520, USA.
²⁶ Department of Biological Chemistry, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
²⁷ Department of Medicine Statistics Core, David Geffen School of Medicine at the University of California, Los Angeles, Los Angeles, CA, USA.
²⁸ Departments of Obstetrics and Gynecology, and Medicine, University of Washington, Seattle, WA, USA.
²⁹ Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
³⁰ Department of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708, USA.
³¹ Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
³² Department of Pathology & Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea.
³³ Department of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, CA 90095, USA.
³⁴ Corrigan Minehan Heart Center and Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USA.
³⁵ Bioinformatics Research Laboratory, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
³⁶ Department of Chemistry, University of California, Davis, CA 95616, USA.
³⁷ Department of Chemical and Biomolecular Engineering and Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.
³⁸ Department of Materials Science & Engineering, University of California Los Angeles, Los Angeles, CA 90095-1595, USA.
³⁹ Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA.
⁴⁰ Program in Quantitative and Computational Biosciences Baylor College of Medicine, Houston, TX 77030, USA.
⁴¹ Department of Medicine, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul, South Korea.
⁴² Scintillon Institute, San Diego, CA, USA.
⁴³ Center for Nanomedicine at the Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
⁴⁴ Department of Veterans Affairs, Surgical Service (112G), San Francisco VA Medical Center, San Francisco, CA 94121, USA.
⁴⁵ Meso Scale Diagnostics, LLC, Rockville, MD 20850, USA.
⁴⁶ Department of Gynecologic Oncology & Reproductive Medicine, University of Texas MD Aderson Cancer Center, Houston, TX 77030, USA.
⁴⁷ Department of Internal Medicine, Hematology/Oncology Division, University of Michigan, Ann Arbor, MI 48109, USA.
⁴⁸ Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
⁴⁹ Center for Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
⁵⁰ Rogel Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA.
⁵¹ Analytical Biochemistry Unit, School of Science, Universidad de la República, Montevideo 11400, Uruguay.
⁵² Cancer Genome Research (B063), German Cancer Research Center DKFZ, Heidelberg 69120, Germany.
⁵³ Heidelberg Biolabs GmbH, Heidelberg 69120, Germany.
⁵⁴ Department of Medicine, Division of Cardiovascular Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
⁵⁵ Department of Obstetrics and Gynecology, University of Washington, Seattle, WA 98195, USA.
⁵⁶ Bioprocess Measurement Group, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
⁵⁷ Institute for Systems Biology, Seattle, WA 98109, USA.
⁵⁸ Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
⁵⁹ Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
⁶⁰ Department of Gynecology, Obstetrics and Gynecologic Oncology, Division of Gynecologic Oncology, Poznan University of Medical Sciences, 60-535 Poznań, Poland.
⁶¹ Department of Nutrition, University of California, Davis, CA 95616, USA.

Abstract

The extracellular RNA communication consortium (ERCC) is an NIH-funded program aiming to promote the development of new technologies, resources, and knowledge about exRNAs and their carriers. After Phase 1 (2013-2018), Phase 2 of the program (ERCC2, 2019-2023) aims to fill critical gaps in knowledge and technology to enable rigorous and reproducible methods for separation and characterization of both bulk populations of exRNA carriers and single EVs. ERCC2 investigators are also developing new bioinformatic pipelines to promote data integration through the exRNA atlas database. ERCC2 has established several Working Groups (Resource Sharing, Reagent Development, Data Analysis and Coordination, Technology Development, nomenclature, and Scientific Outreach) to promote collaboration between ERCC2 members and the broader scientific community. We expect that ERCC2's current and future achievements will significantly improve our understanding of exRNA biology and the development of accurate and efficient exRNA-based diagnostic, prognostic, and theranostic biomarker assays.

Keywords: Biochemistry; Biological sciences; Cell biology; Molecular biology.

Publication types

Review

Abstract

Publication types

Grants and funding