Fixing zein at the fibrillar carboxymethyl cellulose toward an amphiphilic nano-network

Ying Wan; Jian Li; Jitong Ma; Yanan Li; Ren Wang; Zhengxing Chen; Tao Wang

doi:10.1016/j.foodchem.2022.133862

Fixing zein at the fibrillar carboxymethyl cellulose toward an amphiphilic nano-network

Food Chem. 2023 Jan 1:398:133862. doi: 10.1016/j.foodchem.2022.133862. Epub 2022 Aug 6.

Authors

Ying Wan¹, Jian Li², Jitong Ma³, Yanan Li³, Ren Wang³, Zhengxing Chen³, Tao Wang⁴

Affiliations

¹ Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi 214122, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Centre for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
² Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China.
³ Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Centre for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
⁴ Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment and Technology, Jiangnan University, Wuxi 214122, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Centre for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: [email protected].

PMID: 35963220
DOI: 10.1016/j.foodchem.2022.133862

Abstract

In this study, co-assembled protein-polysaccharide complexes (ZCs) were prepared by fixing zein nanoparticles at the fibrillar carboxymethyl cellulose (CMC) by pH-driven anti-solvent precipitation. The complexation boosted the dispersity of zein from 17.3% to 88.6%. Scanning electron microscopy and atomic force microscopy confirmed the formation of network structures where the fibrous polysaccharides inserted into the interval of granular proteins. Circular dichroism spectrum, fluorescence spectrum, and X-ray diffraction verified the electrostatic interaction pattern between zein and CMC. Besides, the ZCs presented favorable amphiphilic properties, and the electrostatic interaction between zein and CMC can be fine-tuned by the substitution degree (DS) of carboxymethyl in CMC. Therefore, the Pickering emulsions stabilized by ZCs had controllable size and long-term stability using DS as a stimulus. Our study offers a novel strategy developing bio-based materials as novel stabilizers of Pickering emulsions.

Keywords: Carboxymethyl cellulose; Electrostatic interactions; Pickering emulsions; Substitution degree; Zein.

MeSH terms

Carboxymethylcellulose Sodium / chemistry
Emulsions / chemistry
Nanoparticles* / chemistry
Particle Size
Zein* / chemistry

Substances

Emulsions
Zein
Carboxymethylcellulose Sodium