Simplified and accessible [18 F]F-AraG synthesis procedure for preclinical PET

Antonia A Högnäsbacka; Miguel A Cortés González; Christer Halldin; Magnus Schou

doi:10.1002/jlcr.3997

Simplified and accessible [¹⁸ F]F-AraG synthesis procedure for preclinical PET

J Labelled Comp Radiopharm. 2022 Aug;65(10-11):288-291. doi: 10.1002/jlcr.3997. Epub 2022 Aug 23.

Authors

Antonia A Högnäsbacka¹, Miguel A Cortés González¹, Christer Halldin¹, Magnus Schou^{1

2}

Affiliations

¹ Department of Clinical Neuroscience, Centre for Psychiatry Research, Karolinska Institutet and Stockholm County Council, Stockholm, Sweden.
² PET Science Centre, Precision Medicine and Biosamples, Oncology R&D, AstraZeneca, Karolinska Institutet, Stockholm, Sweden.

Abstract

The PET tracer [¹⁸ F]F-AraG, an arabinosyl guanine analog, has shown promise for visualizing activated T cells in multiple diseases. Herein, a practitioner's protocol is described, in which the PET tracer is prepared using minimal equipment and manual actions, making it widely accessible for preclinical applications.

Keywords: AraG; SPE purification, T cell imaging; [18F]F-AraG; nucleophilic fluorination; nucleophilic substitution; practitioner's protocol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Guanine
Positron-Emission Tomography* / methods
T-Lymphocytes*

Substances

Guanine