Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants

Hongzhi Wang; Yuxin He; Yingying Wang; Zuren Li; Jiannan Hao; Yijiao Song; Mugui Wang; Jian-Kang Zhu

doi:10.1111/jipb.13352

Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants

J Integr Plant Biol. 2022 Nov;64(11):2029-2032. doi: 10.1111/jipb.13352. Epub 2022 Sep 26.

Authors

Hongzhi Wang^{1

2}, Yuxin He^{1

2}, Yingying Wang^{1

2}, Zuren Li³, Jiannan Hao^{1

2}, Yijiao Song⁴, Mugui Wang¹, Jian-Kang Zhu^{1

5

6}

Affiliations

¹ Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 201602, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
⁴ College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
⁵ Institute of Advanced Biotechnology and School of Life Sciences, Southern University of Science and Technology, Shenzhen, 518055, China.
⁶ Center for Advanced Bioindustry Technologies, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

PMID: 36036619
DOI: 10.1111/jipb.13352

Abstract

Improved cytosine and adenine base editors and an efficient dual editor were applied in targeted evolution of ACETYL COA CARBOXYLASE in rice, resulting in the generation of dozens of herbicide-resistant mutations, at least three of which, W2125L, W2125Q and C2186H, have not been reported previously.

MeSH terms

Acetyl-CoA Carboxylase / genetics
Cytosine
Herbicides* / pharmacology
Mutation / genetics
Oryza* / genetics

Substances

Herbicides
Acetyl-CoA Carboxylase
Cytosine