Intravital microscopy for real-time monitoring of drug delivery and nanobiological processes

Jeffrey Momoh; Dimitrios Kapsokalyvas; Michael Vogt; Sjoerd Hak; Fabian Kiessling; Marc van Zandvoort; Twan Lammers; Alexandros Marios Sofias

doi:10.1016/j.addr.2022.114528

Intravital microscopy for real-time monitoring of drug delivery and nanobiological processes

Adv Drug Deliv Rev. 2022 Oct:189:114528. doi: 10.1016/j.addr.2022.114528. Epub 2022 Sep 5.

Authors

Jeffrey Momoh¹, Dimitrios Kapsokalyvas², Michael Vogt³, Sjoerd Hak⁴, Fabian Kiessling¹, Marc van Zandvoort⁵, Twan Lammers⁶, Alexandros Marios Sofias⁷

Affiliations

¹ Department of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Aachen, Germany.
² Department of Genetics and Cell Biology, Maastricht University, Maastricht, the Netherlands; Interdisciplinary Center for Clinical Research (IZKF), University Hospital RWTH Aachen University, Aachen, Germany.
³ Interdisciplinary Center for Clinical Research (IZKF), University Hospital RWTH Aachen University, Aachen, Germany.
⁴ Department of Biotechnology and Nanomedicine, SINTEF Industry, Trondheim, Norway; Department of Circulation and Medical Imaging, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
⁵ Department of Genetics and Cell Biology, School for Cardiovascular Diseases CARIM, School for Oncology and Reproduction GROW, School for Mental Health and Neuroscience MHeNS, Maastricht University, Maastricht, the Netherlands; Institute for Molecular Cardiovascular Research (IMCAR), University Hospital RWTH Aachen University, Aachen, Germany. Electronic address: [email protected].
⁶ Department of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Aachen, Germany. Electronic address: [email protected].
⁷ Department of Nanomedicine and Theranostics, Institute for Experimental Molecular Imaging, Faculty of Medicine, RWTH Aachen University, Aachen, Germany; Department of Circulation and Medical Imaging, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), Trondheim, Norway; Mildred Scheel School of Oncology (MSSO), Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf (CIO(ABCD)), University Hospital Aachen, Aachen, Germany. Electronic address: [email protected].

PMID: 36067968
DOI: 10.1016/j.addr.2022.114528

Abstract

Intravital microscopy (IVM) expands our understanding of cellular and molecular processes, with applications ranging from fundamental biology to (patho)physiology and immunology, as well as from drug delivery to drug processing and drug efficacy testing. In this review, we highlight modalities, methods and model organisms that make up today's IVM landscape, and we present how IVM - via its high spatiotemporal resolution - enables analysis of metabolites, small molecules, nanoparticles, immune cells, and the (tumor) tissue microenvironment. We furthermore present examples of how IVM facilitates the elucidation of nanomedicine kinetics and targeting mechanisms, as well as of biological processes such as immune cell death, host-pathogen interactions, metabolic states, and disease progression. We conclude by discussing the prospects of IVM clinical translation and examining the integration of machine learning in future IVM practice.

Keywords: Biodistribution; Confocal microscopy; Drug delivery; Drug targeting; Immunotherapy; Intravital microscopy; Multiphoton microscopy; Nanomedicine; Pharmacokinetics; Tumor microenvironment.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Drug Delivery Systems
Humans
Intravital Microscopy*
Neoplasms* / drug therapy
Tumor Microenvironment

Grants and funding

864121/ERC_/European Research Council/International